Influence of 3-Days Introduction of Glutathione on Synthesis of Hydrogen Sulfide in Kidneys under Experimental Nephropathy Conditions Pathology

Author: Kateryna Gerush

Scientific advisor: Bulyk R.Ye. M.D. Department of Medical Biology Bukovinian State Medical University

Kidney diseases are accompanied by metabolic disorders and toxic lesions of human body. Prevention  of  development  of  acute and chronic   kidney  diseases by  the  usage of  effective antioxidants for body  protection from  the  effect  of oxidative  stress in cells is one  of the  most important problems in modern medicine. Hydrogen sulfide is mainly synthesized in kidneys, it is vital for the functioning of the tubular and glomerular kidney apparatus.

The purpose of the  study is to investigate the  influence of glutathione on H2S-system in rat kidneys under experimental nephropathy conditions.

Methods and materials. The experiment was conducted on albino mature rats which weight 160 – 180 g. Nephropathy was modeled by a single intraperitoneal folic acid injection  at a dose of 250 mg/kg. Animals with nephropathy got intragastral glutathione injection  during 3 days at a dose of  100 mg/kg.  Desulfurizing  activity  of  enzymes  and concentration  of  hydrogen  sulfide  were determined in cortical and medullar parts of kidneys.

Results and discussions. During the experiment we have  found the considerable decrease of the  H2S-producing enzymes: cystathionine-β-synthase (CBS) by 24,3%  and cysteine aminotransferase(CAT) by 30,7%  in cortical part of kidneys of rats with experimental nephropathy in comparison with control  group.  These  changes are likely to be  associated with destruction of enzymes due to the increased oxidative modification of proteins, which was observed in the cortical part  of  kidney   under  conditions  of  experimental  nephropathy.  During   the   development  of nephropathy the decrease of concentration of hydrogen sulfide was registered: in medullar part of kidney by 27,8 % and in cortical part by 16% in comparison with control  group.  3-day glutathione introduction made the  H2S-concentration increase to the  norm  in cortical and medullar part of kidney  and increased the  H2S-producing activity of CBS in cortical part of kidney.  As glutathione takes part in supporting redox  potential and detoxification of xenobiotics, its usage is effective during  the  nephropathy. What  is more,  essential structural and functional part of glutathione is amino acid cysteine, which is involved in biosynthesis of hydrogen sulfide.

Our results  show the  necessity of intake of glutathione as a remedy   that  can influence synthesis of hydrogen sulfide for correction and prevention of kidney disease and its complications.

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