Abstract: Immunohistochemical Study of Von Willebrand Factor as a Marker of Endothelial Dysfunctions of Uteroplacental Area and Myometrium in Pregnancy

Authors: Tuleneva Olena, Hoian Anastasiia, Davydenko Ihor
Scientific coordinators: Tuleneva Olena, Davydenko Ihor

Department of Pathological Anatomy

Background: The pathology of cytotrophoblast invasion, endothelial dysfunctions of utero- placental area (UPA) and myometrium lead to placental ischemia and oxidative stress. Among different methods of examination of UPA and myometrium the most effective is morphological
methods, including immunohistochemical staining. Assaying of von Willebrand factor (vWF) permits to study endothelial dysfunctions in different types of blood vessels. VWF is a marker of young endothelial cells, it deposits near the formation of fibrinoid and blood clots (I.Davydenko, 2015).

Aim: To establish the relative immunohistochemical concentration of the vWF in endothelial cells of different types of vessels of the UPA and myometrium in pregnancy.

Materials and Methods: The biopsy material was obtained by means of standard technique.
Multiple paraffin sections (5 mkm thickness) were used for three methods: 1)immunohistochemical vWF staining with an antigen thermal exposure;

2)differentiated histochemical fibrin and collagen staining by N.Z.Slinchenko;

3)hematoxylin-eosin staining. Digital images were analyzed with a
computer program ImageJ (1.48v, W. Rasband, National Institute of Health,USA,2015).

The evaluation of optical density was based on logarithmic transformations of brightness values. We also calculated the arithmetic mean and its error for optical density (PAST3.06, O.Hammer, 2015).

Results: The most pronounced positive colour was observed in formed endothelial cells of the blood vessels, which were stained with varying intensity, in both single blood vessel and different types of blood vessels. The most intense colour was established in arterial type vessels (optical density 0.404±0.0028), and more in myometrium than in the UPA. Less intense colouration was in the venous type vessels (0,381±0,0024) and of the microcirculatory system (0,378±0,0021), however, it did not depend on the localization (myometrium or UPA). The established intensity of colouration of endothelial cells is perspective in studying endothelial dysfunction in vessels of different types, and for arteries – indifferent localization (myometrium or UPA). Moreover, it allows identifying the endothelial cells desquamation as an indicator of huge damage to the intima.

Conclusion: Applying an immunohistochemical study of vWF makes possible to assess the degree and nature of endothelial dysfunction of UPA and myometrium vessels for establishing morphological preconditions for fetal insufficiency.

Keywords: uterine-placental area (UPA); myometrium; pregnancy; von Willebrand factor (vWF).

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